cd138 syndecan 1 microbeads Search Results


cd138 syndecan 1 microbeads  (Miltenyi Biotec)
94
Miltenyi Biotec cd138 syndecan 1 microbeads
Cd138 Syndecan 1 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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direct magnetic labeling with anti cd138 syndecan 1 microbeads  (Miltenyi Biotec)
93
Miltenyi Biotec direct magnetic labeling with anti cd138 syndecan 1 microbeads
Direct Magnetic Labeling With Anti Cd138 Syndecan 1 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd138 positive selection  (Miltenyi Biotec)
96
Miltenyi Biotec cd138 positive selection
Cd138 Positive Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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syndecan  (Miltenyi Biotec)
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Miltenyi Biotec syndecan
Syndecan, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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magnetic cell sorting system  (Miltenyi Biotec)
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Miltenyi Biotec magnetic cell sorting system
Magnetic Cell Sorting System, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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macs column  (Miltenyi Biotec)
97
Miltenyi Biotec macs column
Expression of VH4-34 antibodies in plasma cells from healthy donors and SLE patients. (a) Bone marrow plasma cells from healthy donors were enriched using a <t>MACS</t> <t>CD138</t> column, and the cytoplasmic expression of VH4-34 or VH3 antibodies was detected with anti–light chain and VH-specific anti-Id antibodies 9G4, LJ26, or LC1 (data not shown). In contrast to VH3 and other VH4 antibodies, expression of VH4-34 was absent from normal plasma cells. (b) Identical results were obtained with tonsillar cells. (c) Plasma cells from the peripheral blood of active SLE patients express VH4-34 antibodies with significant frequency (two representative examples are shown). Further analysis revealed that more than 95% of VH4-34 antibodies were of the IgG isotype (data not shown). (d) Differentiation of VH4-34 B cells into plasma cells in vitro. Naive and memory tonsil B cells were sorted and independently cultured in the presence of CD70, IL-2, and IL-10 as described. The frequency of plasma cells was determined by flow cytometry before fractionation (left panel) and after culture (middle panels), and morphological confirmation was obtained by Giemsa staining (right panel). Significant numbers of plasma cells with a CD38Hi/CD20Lo phenotype were obtained with naive and memory cell cultures. (e) Plasma cells obtained in culture were analyzed for intracellular expression of VH4-34 antibodies using FITC-conjugated 9G4 and anti-κ/λ-RPE. VH4-34+ plasma cells were detected at a frequency consistent with the relative abundance of VH4-34 cells in the initial samples and therefore were significantly higher in the naive cell cultures than in the memory cell cultures.
Macs Column, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r-phycoerythrin (pe) conjugated rat anti-mouse cd138 (syndecan-1) monoclonal antibody  (Thermo Fisher)
90
Thermo Fisher r-phycoerythrin (pe) conjugated rat anti-mouse cd138 (syndecan-1) monoclonal antibody
Expression of VH4-34 antibodies in plasma cells from healthy donors and SLE patients. (a) Bone marrow plasma cells from healthy donors were enriched using a <t>MACS</t> <t>CD138</t> column, and the cytoplasmic expression of VH4-34 or VH3 antibodies was detected with anti–light chain and VH-specific anti-Id antibodies 9G4, LJ26, or LC1 (data not shown). In contrast to VH3 and other VH4 antibodies, expression of VH4-34 was absent from normal plasma cells. (b) Identical results were obtained with tonsillar cells. (c) Plasma cells from the peripheral blood of active SLE patients express VH4-34 antibodies with significant frequency (two representative examples are shown). Further analysis revealed that more than 95% of VH4-34 antibodies were of the IgG isotype (data not shown). (d) Differentiation of VH4-34 B cells into plasma cells in vitro. Naive and memory tonsil B cells were sorted and independently cultured in the presence of CD70, IL-2, and IL-10 as described. The frequency of plasma cells was determined by flow cytometry before fractionation (left panel) and after culture (middle panels), and morphological confirmation was obtained by Giemsa staining (right panel). Significant numbers of plasma cells with a CD38Hi/CD20Lo phenotype were obtained with naive and memory cell cultures. (e) Plasma cells obtained in culture were analyzed for intracellular expression of VH4-34 antibodies using FITC-conjugated 9G4 and anti-κ/λ-RPE. VH4-34+ plasma cells were detected at a frequency consistent with the relative abundance of VH4-34 cells in the initial samples and therefore were significantly higher in the naive cell cultures than in the memory cell cultures.
R Phycoerythrin (Pe) Conjugated Rat Anti Mouse Cd138 (Syndecan 1) Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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25g syringe needles  (Becton Dickinson)
90
Becton Dickinson 25g syringe needles
Expression of VH4-34 antibodies in plasma cells from healthy donors and SLE patients. (a) Bone marrow plasma cells from healthy donors were enriched using a <t>MACS</t> <t>CD138</t> column, and the cytoplasmic expression of VH4-34 or VH3 antibodies was detected with anti–light chain and VH-specific anti-Id antibodies 9G4, LJ26, or LC1 (data not shown). In contrast to VH3 and other VH4 antibodies, expression of VH4-34 was absent from normal plasma cells. (b) Identical results were obtained with tonsillar cells. (c) Plasma cells from the peripheral blood of active SLE patients express VH4-34 antibodies with significant frequency (two representative examples are shown). Further analysis revealed that more than 95% of VH4-34 antibodies were of the IgG isotype (data not shown). (d) Differentiation of VH4-34 B cells into plasma cells in vitro. Naive and memory tonsil B cells were sorted and independently cultured in the presence of CD70, IL-2, and IL-10 as described. The frequency of plasma cells was determined by flow cytometry before fractionation (left panel) and after culture (middle panels), and morphological confirmation was obtained by Giemsa staining (right panel). Significant numbers of plasma cells with a CD38Hi/CD20Lo phenotype were obtained with naive and memory cell cultures. (e) Plasma cells obtained in culture were analyzed for intracellular expression of VH4-34 antibodies using FITC-conjugated 9G4 and anti-κ/λ-RPE. VH4-34+ plasma cells were detected at a frequency consistent with the relative abundance of VH4-34 cells in the initial samples and therefore were significantly higher in the naive cell cultures than in the memory cell cultures.
25g Syringe Needles, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5×10 −5 m β-2-mercaptoethanol  (Fisher Scientific)
90
Fisher Scientific 5×10 −5 m β-2-mercaptoethanol
Expression of VH4-34 antibodies in plasma cells from healthy donors and SLE patients. (a) Bone marrow plasma cells from healthy donors were enriched using a <t>MACS</t> <t>CD138</t> column, and the cytoplasmic expression of VH4-34 or VH3 antibodies was detected with anti–light chain and VH-specific anti-Id antibodies 9G4, LJ26, or LC1 (data not shown). In contrast to VH3 and other VH4 antibodies, expression of VH4-34 was absent from normal plasma cells. (b) Identical results were obtained with tonsillar cells. (c) Plasma cells from the peripheral blood of active SLE patients express VH4-34 antibodies with significant frequency (two representative examples are shown). Further analysis revealed that more than 95% of VH4-34 antibodies were of the IgG isotype (data not shown). (d) Differentiation of VH4-34 B cells into plasma cells in vitro. Naive and memory tonsil B cells were sorted and independently cultured in the presence of CD70, IL-2, and IL-10 as described. The frequency of plasma cells was determined by flow cytometry before fractionation (left panel) and after culture (middle panels), and morphological confirmation was obtained by Giemsa staining (right panel). Significant numbers of plasma cells with a CD38Hi/CD20Lo phenotype were obtained with naive and memory cell cultures. (e) Plasma cells obtained in culture were analyzed for intracellular expression of VH4-34 antibodies using FITC-conjugated 9G4 and anti-κ/λ-RPE. VH4-34+ plasma cells were detected at a frequency consistent with the relative abundance of VH4-34 cells in the initial samples and therefore were significantly higher in the naive cell cultures than in the memory cell cultures.
5×10 −5 M β 2 Mercaptoethanol, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5×10 −5 m β-2-mercaptoethanol/product/Fisher Scientific
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β-2-mercaptoethanol  (Fisher Scientific)
90
Fisher Scientific β-2-mercaptoethanol
Expression of VH4-34 antibodies in plasma cells from healthy donors and SLE patients. (a) Bone marrow plasma cells from healthy donors were enriched using a <t>MACS</t> <t>CD138</t> column, and the cytoplasmic expression of VH4-34 or VH3 antibodies was detected with anti–light chain and VH-specific anti-Id antibodies 9G4, LJ26, or LC1 (data not shown). In contrast to VH3 and other VH4 antibodies, expression of VH4-34 was absent from normal plasma cells. (b) Identical results were obtained with tonsillar cells. (c) Plasma cells from the peripheral blood of active SLE patients express VH4-34 antibodies with significant frequency (two representative examples are shown). Further analysis revealed that more than 95% of VH4-34 antibodies were of the IgG isotype (data not shown). (d) Differentiation of VH4-34 B cells into plasma cells in vitro. Naive and memory tonsil B cells were sorted and independently cultured in the presence of CD70, IL-2, and IL-10 as described. The frequency of plasma cells was determined by flow cytometry before fractionation (left panel) and after culture (middle panels), and morphological confirmation was obtained by Giemsa staining (right panel). Significant numbers of plasma cells with a CD38Hi/CD20Lo phenotype were obtained with naive and memory cell cultures. (e) Plasma cells obtained in culture were analyzed for intracellular expression of VH4-34 antibodies using FITC-conjugated 9G4 and anti-κ/λ-RPE. VH4-34+ plasma cells were detected at a frequency consistent with the relative abundance of VH4-34 cells in the initial samples and therefore were significantly higher in the naive cell cultures than in the memory cell cultures.
β 2 Mercaptoethanol, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hanks bss (no ca 2+ and mg 2+)  (Fisher Scientific)
90
Fisher Scientific hanks bss (no ca 2+ and mg 2+)
Expression of VH4-34 antibodies in plasma cells from healthy donors and SLE patients. (a) Bone marrow plasma cells from healthy donors were enriched using a <t>MACS</t> <t>CD138</t> column, and the cytoplasmic expression of VH4-34 or VH3 antibodies was detected with anti–light chain and VH-specific anti-Id antibodies 9G4, LJ26, or LC1 (data not shown). In contrast to VH3 and other VH4 antibodies, expression of VH4-34 was absent from normal plasma cells. (b) Identical results were obtained with tonsillar cells. (c) Plasma cells from the peripheral blood of active SLE patients express VH4-34 antibodies with significant frequency (two representative examples are shown). Further analysis revealed that more than 95% of VH4-34 antibodies were of the IgG isotype (data not shown). (d) Differentiation of VH4-34 B cells into plasma cells in vitro. Naive and memory tonsil B cells were sorted and independently cultured in the presence of CD70, IL-2, and IL-10 as described. The frequency of plasma cells was determined by flow cytometry before fractionation (left panel) and after culture (middle panels), and morphological confirmation was obtained by Giemsa staining (right panel). Significant numbers of plasma cells with a CD38Hi/CD20Lo phenotype were obtained with naive and memory cell cultures. (e) Plasma cells obtained in culture were analyzed for intracellular expression of VH4-34 antibodies using FITC-conjugated 9G4 and anti-κ/λ-RPE. VH4-34+ plasma cells were detected at a frequency consistent with the relative abundance of VH4-34 cells in the initial samples and therefore were significantly higher in the naive cell cultures than in the memory cell cultures.
Hanks Bss (No Ca 2+ And Mg 2+), supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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falcon polystyrene 5ml round bottom tubes  (Becton Dickinson)
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Becton Dickinson falcon polystyrene 5ml round bottom tubes
Expression of VH4-34 antibodies in plasma cells from healthy donors and SLE patients. (a) Bone marrow plasma cells from healthy donors were enriched using a <t>MACS</t> <t>CD138</t> column, and the cytoplasmic expression of VH4-34 or VH3 antibodies was detected with anti–light chain and VH-specific anti-Id antibodies 9G4, LJ26, or LC1 (data not shown). In contrast to VH3 and other VH4 antibodies, expression of VH4-34 was absent from normal plasma cells. (b) Identical results were obtained with tonsillar cells. (c) Plasma cells from the peripheral blood of active SLE patients express VH4-34 antibodies with significant frequency (two representative examples are shown). Further analysis revealed that more than 95% of VH4-34 antibodies were of the IgG isotype (data not shown). (d) Differentiation of VH4-34 B cells into plasma cells in vitro. Naive and memory tonsil B cells were sorted and independently cultured in the presence of CD70, IL-2, and IL-10 as described. The frequency of plasma cells was determined by flow cytometry before fractionation (left panel) and after culture (middle panels), and morphological confirmation was obtained by Giemsa staining (right panel). Significant numbers of plasma cells with a CD38Hi/CD20Lo phenotype were obtained with naive and memory cell cultures. (e) Plasma cells obtained in culture were analyzed for intracellular expression of VH4-34 antibodies using FITC-conjugated 9G4 and anti-κ/λ-RPE. VH4-34+ plasma cells were detected at a frequency consistent with the relative abundance of VH4-34 cells in the initial samples and therefore were significantly higher in the naive cell cultures than in the memory cell cultures.
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Image Search Results


Expression of VH4-34 antibodies in plasma cells from healthy donors and SLE patients. (a) Bone marrow plasma cells from healthy donors were enriched using a MACS CD138 column, and the cytoplasmic expression of VH4-34 or VH3 antibodies was detected with anti–light chain and VH-specific anti-Id antibodies 9G4, LJ26, or LC1 (data not shown). In contrast to VH3 and other VH4 antibodies, expression of VH4-34 was absent from normal plasma cells. (b) Identical results were obtained with tonsillar cells. (c) Plasma cells from the peripheral blood of active SLE patients express VH4-34 antibodies with significant frequency (two representative examples are shown). Further analysis revealed that more than 95% of VH4-34 antibodies were of the IgG isotype (data not shown). (d) Differentiation of VH4-34 B cells into plasma cells in vitro. Naive and memory tonsil B cells were sorted and independently cultured in the presence of CD70, IL-2, and IL-10 as described. The frequency of plasma cells was determined by flow cytometry before fractionation (left panel) and after culture (middle panels), and morphological confirmation was obtained by Giemsa staining (right panel). Significant numbers of plasma cells with a CD38Hi/CD20Lo phenotype were obtained with naive and memory cell cultures. (e) Plasma cells obtained in culture were analyzed for intracellular expression of VH4-34 antibodies using FITC-conjugated 9G4 and anti-κ/λ-RPE. VH4-34+ plasma cells were detected at a frequency consistent with the relative abundance of VH4-34 cells in the initial samples and therefore were significantly higher in the naive cell cultures than in the memory cell cultures.

Journal:

Article Title: Regulation of inherently autoreactive VH4-34 B cells in the maintenance of human B cell tolerance

doi:

Figure Lengend Snippet: Expression of VH4-34 antibodies in plasma cells from healthy donors and SLE patients. (a) Bone marrow plasma cells from healthy donors were enriched using a MACS CD138 column, and the cytoplasmic expression of VH4-34 or VH3 antibodies was detected with anti–light chain and VH-specific anti-Id antibodies 9G4, LJ26, or LC1 (data not shown). In contrast to VH3 and other VH4 antibodies, expression of VH4-34 was absent from normal plasma cells. (b) Identical results were obtained with tonsillar cells. (c) Plasma cells from the peripheral blood of active SLE patients express VH4-34 antibodies with significant frequency (two representative examples are shown). Further analysis revealed that more than 95% of VH4-34 antibodies were of the IgG isotype (data not shown). (d) Differentiation of VH4-34 B cells into plasma cells in vitro. Naive and memory tonsil B cells were sorted and independently cultured in the presence of CD70, IL-2, and IL-10 as described. The frequency of plasma cells was determined by flow cytometry before fractionation (left panel) and after culture (middle panels), and morphological confirmation was obtained by Giemsa staining (right panel). Significant numbers of plasma cells with a CD38Hi/CD20Lo phenotype were obtained with naive and memory cell cultures. (e) Plasma cells obtained in culture were analyzed for intracellular expression of VH4-34 antibodies using FITC-conjugated 9G4 and anti-κ/λ-RPE. VH4-34+ plasma cells were detected at a frequency consistent with the relative abundance of VH4-34 cells in the initial samples and therefore were significantly higher in the naive cell cultures than in the memory cell cultures.

Article Snippet: Bone marrow and tonsillar mononuclear cells isolated through a Ficoll-Paque gradient were labeled with CD138 (Syndecan-1) microbeads and positively selected on a MACS column (Miltenyi Biotec).

Techniques: Expressing, Clinical Proteomics, In Vitro, Cell Culture, Flow Cytometry, Fractionation, Staining